Asian Journal of Research in Chemistry

V. Veeranna ¹, V. Suryanarayana Rao ¹

Affiliations
1 Department of Chemistry, Sri Krishnadevaraya University, Anantapur -515003, AP., IN

Abstract

A simple, selective and sensitive second order derivative spectrophotometric method is developed using FFTSC (furfuraldehyde thiosemicarbazone) for the simultaneous determination of Hg(II) and Co(II). The reagent FFTSC gives yellow colour with Hg(II) and Co(II) mixture solution in basic medium. The maximum peaks were observed in between 360nm - 420nm for both Hg(II) and Co(II) in basic buffer solution of pH 8.5. The molar absorptivity and sandell`s sensitivity of Hg(II) and Co(II)-FFTSC complexes are 2.2x10⁴ L/mol/cm, 4.3x10³ L/mol/cm and 0.000454 μg/cm², 0.00232 μg/cm² respectively. The stability constant of Hg (II) and Co(II)-FFTSC complexes are 3.495x10⁶ and 5.176x10⁴ respectively. The effect of concentrations of Hg(II) and Co(II) ions on amplitude are also studied. The samples of tap water, distilled water, human tissues and blood samples were analyzed by the proposed method. The results of the samples are good agreement with the certified reference values APARI, CVAAT and AAS methods.

Keywords

FFTSC, Simultaneous Spectrophotometry, Mercury (II), Cobalt (II).

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A. Raghavendra Guru Prasad ¹, V. Suryanarayana Rao ²

Affiliations
1 Department of Chemistry, ICFAI University, Dehradun, U.K., IN
2 Department of Chemistry, Sri Krishnadevaraya University, Anantapur, IN

Abstract

Simple, selective and sensitive first order spectrophotometric method has been developed for the determination of hostacycline and oxytetracycline at pH 3.5. Linear calibration plots are obtained in the range of 10 to 60 μg/ml for each of the tetracycline. The method is also extended for the resolution of their binary mixtures by using the zero-crossing method for evaluation of the derivative signal. The corresponding calibration plot is linear in the rage 10 μg/ml to 25 μg/ml for each of the tetracycline. Regression equation, correlation coefficient and percentage recovery is reported in each case. The developed method has been successfully applied for the determination of hostacycline and oxytetracycline in pharmaceutical formulations, urine and honey. A notable advantage of the proposed method is that the method can be applied without any pretreatment of the samples.

Keywords

Hostacycline, Oxytetracycline, Spectrophotometry, Zero Crossing Method, Resolution.

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